Strain-Promoted “Click” Chemistry for Terminal Labeling of DNA
نویسندگان
چکیده
منابع مشابه
Site-specific terminal and internal labeling of RNA by poly(A) polymerase tailing and copper-catalyzed or copper-free strain-promoted click chemistry
The modification of RNA with fluorophores, affinity tags and reactive moieties is of enormous utility for studying RNA localization, structure and dynamics as well as diverse biological phenomena involving RNA as an interacting partner. Here we report a labeling approach in which the RNA of interest--of either synthetic or biological origin--is modified at its 3'-end by a poly(A) polymerase wit...
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Site-specific labeling of biomolecules is rapidly advancing due to the discovery of novel mutually orthogonal reactions. Quantum chemistry studies have also increased our understanding of their relative rates, although these have until now been based on highly simplified reactants. Here we examine a set of strain-promoted click-type cycloaddition reactions of n-propyl azide, 3-benzyl tetrazine ...
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A facile and robust approach is provided for the synthesis of highly dispersed copper nanoparticles immobilized onto graphene nanosheets, useful as a recyclable and reusable heterogeneous catalyst with excellent catalytic activity to achieve Cu(I)-catalyzed [3+2] cycloaddition 'click' chemistry.
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Specific and highly efficient fluorescent labeling techniques for biomolecules, especially for proteins, are required for the quantitative analyses of bio-phenomena and for subsequent systems biology. Although expression of exogenous proteins fused with fluorescent tags, such as green fluorescent protein, is the most widely used method for quantitative bio-analysis, the following problems need ...
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ژورنال
عنوان ژورنال: Bioconjugate Chemistry
سال: 2011
ISSN: 1043-1802,1520-4812
DOI: 10.1021/bc1003668